Figure 3From: Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in miceScreening of the suitable silkworm strain for PEDV S protein expression. A The Western blotting analysis of 25 silkworm strains infected with recombinant baculovirus encoding PEDV/S(1–1320) + CMP + Tags. The sera of 0.5 μL were subjected to 8% SDS-PAGE, and the specific band was detected using HisProbe-HRP. Three individual samples were prepared from each strain, and f38 sera were used as a control. B Comparison of relative band intensities among silkworm strains by Western blotting detecting PEDV/S(1–1320) + CMP + Tags protein. The intensities of each band were measured, and the relative intensities were calculated using the average band intensity of f38 strain as 1. Bars represent mean relative intensities ± SE (N = 3). C Expression analysis of the hybrid silkworm of n22 strain and xa50 strain. Three individual serum samples of each n22 (lane1-3), xa50 (lane4-6), and n22 × xa50 (lane7-9), and xa50 × n22 (lane10-12) were resolved in an 8% SDS-PAGE with CBB stain (upper panel) and Western blotting using HisProbe-HRP (lower panel). Orange arrows indicate the PEDV S protein.Back to article page