Figure 2From: Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in miceGenetic fusion of trimerization motifs to the S protein. A, B Scheme of PEDV S protein fused with the trimeric motif. The CMP coiled-coil was appended at the C terminus of the S protein. C Expression analysis of the S protein fused with the trimeric motif. Medium: culture medium; Soluble: soluble samples of cell lysates; Insoluble: insoluble samples of cell lysates. All samples from BmO2 cells (5 μL) and silkworm sera (0.5 μL) were resolved on an 8% SDS-PAGE. The recombinant PEDV S protein was also detected by Western blotting using HisProbe-HRP. Orange arrows indicate the PEDV S protein.Back to article page