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Figure 2 | Veterinary Research

Figure 2

From: Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in mice

Figure 2

Genetic fusion of trimerization motifs to the S protein. A, B Scheme of PEDV S protein fused with the trimeric motif. The CMP coiled-coil was appended at the C terminus of the S protein. C Expression analysis of the S protein fused with the trimeric motif. Medium: culture medium; Soluble: soluble samples of cell lysates; Insoluble: insoluble samples of cell lysates. All samples from BmO2 cells (5 μL) and silkworm sera (0.5 μL) were resolved on an 8% SDS-PAGE. The recombinant PEDV S protein was also detected by Western blotting using HisProbe-HRP. Orange arrows indicate the PEDV S protein.

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