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Table 1 Bacterial strains and plasmids.

From: DctR contributes to the virulence of avian pathogenic Escherichia coli through regulation of type III secretion system 2 expression

Strains or plasmids Characteristics References
Strains
 CE08 APEC wild-type strain, O2: K1 serotype This study
 ΔdctR dctR deletion mutant in CE08 This study
 CΔdctR ΔdctR with plasmid pSTV28-dctR This study
 DH5α F-, Δ(lacZYA-argF)U169, recA1, endA1, hsdR17(rk-, mk +), phoA, supE44, λ- TIANGEN
Plasmids
 pSTV28-Pamp Cm, lacZ, Pamp promoter This study
 pSTV28-dctR pSTV28-Pamp derivative harboring dctR gene This study
 pKD46 Amp; expresses λ red recombinase [24]
 pKD3 Cm, template plasmid [24]
 pCP20 Cm, Amp, yeast Flp recombinase gene, FLP [24]