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Figure 7 | Veterinary Research

Figure 7

From: DctR contributes to the virulence of avian pathogenic Escherichia coli through regulation of type III secretion system 2 expression

Figure 7

Determination of inflammatory cytokine expressions in avian macrophages HD-11. HD-11 cells were infected with each APEC strain. The transcription levels of IL-1β and IL-8 in bacteria-infected HD-11 cells were analyzed at 3 h, 6 h post-invasion by qRT-PCR. APEC infection led to significantly upregulated expressions of inflammatory cytokines IL-1β and IL-8 compared to the uninfected cells. The expression of IL-1β and IL-8 was significantly decreased in HD-11 cells infected with ΔdctR compared with the wild-type strain at 6 h post-invasion. Statistical significance was assessed by two-way ANOVA (*P < 0.05).

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Veterinary Research

ISSN: 1297-9716