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Mutagenesis
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pCD163-G499R, forward
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GTACAACATGGAGACACGTGGcgcACCGTCTGTGATTCTGAC
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pCD163-G499R, reverse
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GTCAGAATCACAGACGGTgcgCCACGTGTCTCCATGTTGTAC
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pCD163-E509H, forward
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GTGATTCTGACTTCTCTCTGcacGCGGCCAGCGTGCTGTGC
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pCD163-E509H, reverse
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GCACAGCACGCTGGCCGCgtgCAGAGAGAAGTCAGAATCAC
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pCD163-S512N, forward
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GACTTCTCTCTGGAGGCGGCCaacGTGCTGTGCAGGGAACTAC
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pCD163-S512N, reverse
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GTAGTTCCCTGCACAGCACgttGGCCGCCTCCAGAGAGAAGTC
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pCD163-T522D, forward
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GAACTACAGTGCGGCgacGTGGTTTCCCTC
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pCD163-T522D, reverse
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CAGGAGGGAAACCACgtcGCCGCACTGTAGTTC
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pCD163-E534K, forward
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CTGGGGGGAGCTCACTTTGGAaaaGGAAGTGGACAGATCTG
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pCD163-E534K, reverse
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CAGATCTGTCCACTTCCtttTCCAAAGTGAGCTCCCCCCAG
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pCD163-E543K, forward
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GTGGACAGATCTGGGCTGAAaaaTTCCAGTGTGAGGGGCACGAG
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pCD163-E543K, reverse
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CTCGTGCCCCTCACACTGGAAtttTTCAGCCCAGATCTGTCCAC
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pCD163-H549S, forward
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GAAGAATTCCAGTGTGAGGGGagcGAGTCCCACCTTTCACTCTG
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pCD163-H549S, reverse
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CAGAGTGAAAGGTGGGACTCgctCCCCTCACACTGGAATTCTTC
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pCD163-P560Q, forward
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CTTTCACTCTGCCCAGTAGCAcaaCGCCCTGACGGGACATGTAGC
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pCD163-P560Q, reverse
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GCTACATGTCCCGTCAGGGCGttgTGCTACTGGGCAGAGTGAAAG
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pCD163-R561H, forward
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CACTCTGCCCAGTAGCACCCcacCCTGACGGGACATGTAGCCAC
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pCD163-R561H, reverse
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GTGGCTACATGTCCCGTCAGGgtgGGGTGCTACTGGGCAGAGTG
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pCD163-G564D, forward
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CAGTAGCACCCCGCCCTGACgacACATGTAGCCACAGCAGGGAC
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pCD163-G564D, reverse
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GTCCCTGCTGTGGCTACATGTgtcGTCAGGGCGGGGTGCTACTG
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pCD163-E534K/R561H, forward
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CGGCGTAGTCTGCTCAAGATACACACAAATC
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pCD163-E534K/R561H, reverse
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CTCCAACCAGCCTGGGTTTCCTGTGGGCTG
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RT-qPCR
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PRRSV-ORF7, forward
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AAACCAGTCCAGAGGCAAGG
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PRRSV-ORF7, reverse
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GCAAACTAAACTCCACAGTGTAA
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GAPDH, forward
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CCTTCCGTGTCCCTACTGCCAAC
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GAPDH, reverse
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GACGCCTGCTTCACCACCTTCT
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