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Table 2 Primers for mutagenesis and RT-qPCR in this study

From: Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro

Primer

Sequencea

Mutagenesis

 pCD163-G499R, forward

GTACAACATGGAGACACGTGGcgcACCGTCTGTGATTCTGAC

 pCD163-G499R, reverse

GTCAGAATCACAGACGGTgcgCCACGTGTCTCCATGTTGTAC

 pCD163-E509H, forward

GTGATTCTGACTTCTCTCTGcacGCGGCCAGCGTGCTGTGC

 pCD163-E509H, reverse

GCACAGCACGCTGGCCGCgtgCAGAGAGAAGTCAGAATCAC

 pCD163-S512N, forward

GACTTCTCTCTGGAGGCGGCCaacGTGCTGTGCAGGGAACTAC

 pCD163-S512N, reverse

GTAGTTCCCTGCACAGCACgttGGCCGCCTCCAGAGAGAAGTC

 pCD163-T522D, forward

GAACTACAGTGCGGCgacGTGGTTTCCCTC

 pCD163-T522D, reverse

CAGGAGGGAAACCACgtcGCCGCACTGTAGTTC

 pCD163-E534K, forward

CTGGGGGGAGCTCACTTTGGAaaaGGAAGTGGACAGATCTG

 pCD163-E534K, reverse

CAGATCTGTCCACTTCCtttTCCAAAGTGAGCTCCCCCCAG

 pCD163-E543K, forward

GTGGACAGATCTGGGCTGAAaaaTTCCAGTGTGAGGGGCACGAG

 pCD163-E543K, reverse

CTCGTGCCCCTCACACTGGAAtttTTCAGCCCAGATCTGTCCAC

 pCD163-H549S, forward

GAAGAATTCCAGTGTGAGGGGagcGAGTCCCACCTTTCACTCTG

 pCD163-H549S, reverse

CAGAGTGAAAGGTGGGACTCgctCCCCTCACACTGGAATTCTTC

 pCD163-P560Q, forward

CTTTCACTCTGCCCAGTAGCAcaaCGCCCTGACGGGACATGTAGC

 pCD163-P560Q, reverse

GCTACATGTCCCGTCAGGGCGttgTGCTACTGGGCAGAGTGAAAG

 pCD163-R561H, forward

CACTCTGCCCAGTAGCACCCcacCCTGACGGGACATGTAGCCAC

 pCD163-R561H, reverse

GTGGCTACATGTCCCGTCAGGgtgGGGTGCTACTGGGCAGAGTG

 pCD163-G564D, forward

CAGTAGCACCCCGCCCTGACgacACATGTAGCCACAGCAGGGAC

 pCD163-G564D, reverse

GTCCCTGCTGTGGCTACATGTgtcGTCAGGGCGGGGTGCTACTG

 pCD163-E534K/R561H, forward

CGGCGTAGTCTGCTCAAGATACACACAAATC

 pCD163-E534K/R561H, reverse

CTCCAACCAGCCTGGGTTTCCTGTGGGCTG

RT-qPCR

 PRRSV-ORF7, forward

AAACCAGTCCAGAGGCAAGG

 PRRSV-ORF7, reverse

GCAAACTAAACTCCACAGTGTAA

 GAPDH, forward

CCTTCCGTGTCCCTACTGCCAAC

 GAPDH, reverse

GACGCCTGCTTCACCACCTTCT

  1. aThe lowercase letters indicate mutated sites.