Figure 4

IFA analyses of WT and mutant pCD163 expression. A Sequence alignment of pCD163 SRCR5, moCD163 SRCR5 and hCD163L1 SRCR8. The amino acid sequences of pCD163 SRCR5 (UniProt entry Q2VL90), moCD163 SRCR5 (UniProt entry Q2VLG4) and hCD163L1 SRCR8 (UniProt entry Q9NR16) are aligned. The chosen mutated residues are marked by asterisks (*). B IFA analyses of WT and mutant pCD163 expression. The PK-15 cells were transfected with WT or pCD163 mutated constructs (1 μg/well, 24-well plate). Twenty four hours post-transfection, the cells were fixed and stained with a commercial mouse anti-pCD163 monoclonal antibody (MCA2311GA), and then examined by IFA. The total fluorescence intensity of pCD163 was calculated using ImageJ software. Data represent means ± SEM of three independent experiments. ns, not significant.