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Figure 3 | Veterinary Research

Figure 3

From: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis

Figure 3

Immunogens analysis of mAb 2D1. A Reaction of G. parasuis with mAb 2D1 was identified by Western blot. M: Protein markers; lanes 1 and 2: G. parasuis serovar 5 strain (the primary antibodies for lane 1 were mAb 2D1 and for lane 2 were mAb ApxIV). B The expression of recombinant proteins was analyzed by SDS-PAGE. M1, M2, M3: Protein markers, lane 1: Rosetta containing pET-32a induced by IPTG; lanes 2 and 3: Rosetta containing pET-32a-porin induced by IPTG (the arrow indicates the position of the protein); lanes 4 and 5: Rosetta containing pET-28a-Fe(3+) ABC-transporter substrate-binding protein induced by IPTG; lane 6: Rosetta containing pET-28a induced by IPTG; lanes 7 and 8: Rosetta containing pET-28a-GAPDH induced by IPTG; lane 9: Rosetta containing pET-28a induced by IPTG (all were done with mAb 2D1 as the primary antibody). C The reactions of recombinant proteins with mAb 2D1 were analyzed by Western blot. Lane 1: Rosetta containing pET-32a induced by IPTG; lane 2: Rosetta containing pET-32a-porin induced by IPTG; lane 3: Rosetta containing pET-28a-GAPDH induced by IPTG; lane 4, 7: Rosetta containing pET-28a induced by IPTG; lanes 5 and 6: Rosetta containing pET-28a-Fe(3+) ABC-transporter substrate-binding protein induced by IPTG (all were done with mAb 2D1 as the primary antibody); lane 8: Rosetta containing pET-28a-Fe(3+) ABC-transporter substrate-binding protein induced by IPTG; lane 9: Rosetta containing pET-28a induced by IPTG (the primary antibody of lane 8 and lane 9 is mAb ApxIV).

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