Table 1 Primers used for quantitative real-time PCR
Gene name | Sense (5’-3’)-forward | Antisense (5’-3’)-reverse |
|---|---|---|
GAPDH | TCGGAGTGAACGGATTTGGC | TGACAAGCTTCCCGTTCTCC |
ZO1 | AGGCGTGTTTAACAGCAACG | CCAAAGGACTCAGCAGGGTT |
OCLN | TCGTCCAACGGGAAAGTGAA | ATCAGTGGAAGTTCCTGAACCA |
CLDN1 | TGGAAGATTTACTCCTACGCTGG | TCCCAGCAGGATGCCAATTACC |
MUC2 | GGACGCCTACAAGGAGTTCG | ACCAGCTGCTGAGTGAGGTA |
PRRSV N Gene | AGAAGCCCCATTTCCCTCTA | CGGATCAGACGCACAGTATG |
IL-1β | CCCAAAAGTTACCCGAAGAGG | TCTGCTTGAGAGGTGCTGATG |
IL-6 | ACAAAGCCACCACCCCTAAC | CGTGGACGGCATCAATCTCA |
IL-8 | TTCACAAGTCTCTGCTCAACTG | TGTCCTCAAGGTAGGATGGG |
TNF-α | CCCTCACGTCCTTCTGGTTT | GAGTCTGGAAGCCCCAGTTC |