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Table 1 Sequences of the primers and sgRNAs utilized in this study

From: CRISPR/Cas9-based generation of a recombinant double-reporter pseudorabies virus and its characterization in vitro and in vivo

Primers and sgRNAs Sequences (5'–3')
US 6/7-F 5'-GAATTCCGGCGTGAACATCCTCACCGACT-3'
US 6/7-R 5'-GATATCGCAGCGTCCCGTCTATCGTCAGGT-3'
US 8/9-F 5'-GGATCCGCCCACGCACGAGGACTACTACGA-3'
US 8/9-R 5'-GCTAGCGGTGGAGGCGGTGGAGAAGAAGAG-3'
sgRNA1-F 5'-CACCGTCGTGCCACGATCCGACGA-3'
sgRNA1-R 5'-AAACTCGTCGGATCGTGGCACGAC-3'
sgRNA2-F 5'-CACCGTACAGCCCCGACTCGTCCG-3'
sgRNA2-R 5'-AAACCGGACGAGTCGGGGCTGTAC-3'
U6-F 5'-CTATTTCCCATGATTCCTTCA-3'
CAG-screen R 5'-GTACTGGGCACAATGCCAG-3'
Porcine actin-F 5'-CTGAACCCCAAAGCCAACCGT-3'
Porcine actin-R 5'-TTCTCCTTGATGTCCCGCACG-3'
Porcine IFN-β-F 5'-AGTTGCCTGGGACTCCTCAA -3'
Porcine IFN-β-R 5'-CCTCAGGGACCTCAAAGTTCAT-3'
Porcine IL-1β-F 5'-CCATCCACTGAGCCAGCCTT-3'
Porcine IL-1β-R 5'-TGCCAAGGACAGAGGACTGC-3'
Luc-F 5'-ATGGAAGACGCCAAAAACATAAAG-3'
Luc-R 5'-TTACACGGCGATCTTTCCGCCCT-3'