Table 1 Sequences of the primers and sgRNAs utilized in this study
Primers and sgRNAs | Sequences (5'–3') |
|---|---|
US 6/7-F | 5'-GAATTCCGGCGTGAACATCCTCACCGACT-3' |
US 6/7-R | 5'-GATATCGCAGCGTCCCGTCTATCGTCAGGT-3' |
US 8/9-F | 5'-GGATCCGCCCACGCACGAGGACTACTACGA-3' |
US 8/9-R | 5'-GCTAGCGGTGGAGGCGGTGGAGAAGAAGAG-3' |
sgRNA1-F | 5'-CACCGTCGTGCCACGATCCGACGA-3' |
sgRNA1-R | 5'-AAACTCGTCGGATCGTGGCACGAC-3' |
sgRNA2-F | 5'-CACCGTACAGCCCCGACTCGTCCG-3' |
sgRNA2-R | 5'-AAACCGGACGAGTCGGGGCTGTAC-3' |
U6-F | 5'-CTATTTCCCATGATTCCTTCA-3' |
CAG-screen R | 5'-GTACTGGGCACAATGCCAG-3' |
Porcine actin-F | 5'-CTGAACCCCAAAGCCAACCGT-3' |
Porcine actin-R | 5'-TTCTCCTTGATGTCCCGCACG-3' |
Porcine IFN-β-F | 5'-AGTTGCCTGGGACTCCTCAA -3' |
Porcine IFN-β-R | 5'-CCTCAGGGACCTCAAAGTTCAT-3' |
Porcine IL-1β-F | 5'-CCATCCACTGAGCCAGCCTT-3' |
Porcine IL-1β-R | 5'-TGCCAAGGACAGAGGACTGC-3' |
Luc-F | 5'-ATGGAAGACGCCAAAAACATAAAG-3' |
Luc-R | 5'-TTACACGGCGATCTTTCCGCCCT-3' |