Development of porcine enteroids from small intestinal crypts. A Enteroids cultured using IESC or (B) L-WRN-conditioned IESC medium, respectively. Images were taken from day 1 until day 14 and are representative for enteroid development from jejunal crypts of 9 piglets. C Enteroids cultured using Intesticult organoid growth medium were followed from day 0 until day 4 and are representative for enteroid development from jejunal crypts of 4 piglets. D Comparison of enteroid cultures originating from duodenal, jejunal and ileal crypts. Images represent enteroids 6 days after passaging and are representative of enteroid cultures from crypts obtained from duodenum (n = 4), jejunum (n = 10) and ileum (n = 8). Scale bar equals 100 µm.