Figure 3

Phenotypic profile of Canaria Hair Breed (CHB) and Canaria Sheep (CS) abomasal lymph node (ALN) cells following vaccination with a prototype recombinant sub-unit Teladorsagia circumcincta vaccine and subsequent challenge with T. circumcincta. The expression of cell surface markers corresponding to main blood cell lineages: T helper CD4⁺ cells, cytotoxic CD8⁺ T cells and γδ⁺ T cells, NK cells, CD21⁺ B cells and CD14⁺ myeloid cells (monocytes and macrophages) were examined by single colour flow cytometry. Cells were gated to eliminate dead cells and doublets. Threshold levels which determined positivity for the selected cell surface markers were set with secondary antibody only. Results are shown as boxplots with IQR and error bars indicating ± SE. “*” indicates statistical significance for p < 0.05, “**” p < 0.01 and “***” p < 0.001. Abbreviations: Vac  Vaccinated, Con  Control (adjuvant-only).