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Table 2 Details of the experiments: dose, mode of exposure and type of inoculum used in each experiment

From: Transmission of infectious laryngotracheitis virus vaccine and field strains: the role of degree of contact and transmission by whole blood, plasma and poultry dust

Experiment Treatment Isolator (n) Chickens/isolator (n) ILTV strain Inoculum Mode of exposure Dose Age at exposure (d)
Expt. 1 CONTACT Eye drop (donors) 3 8 A20 Serva
C9
Virus Eye drop a103.5 PFU/bird (A20)
102.8 EID/bird
(Serva)
104TCID50/bird (Class 9)
14
In-contact 3 8 A20 Serva
C9
Nil Contact with donors
Shared air space 3 16 A20 Serva
C9
Nil Shared air with donors
Expt. 2 DUST INF 3 dpe dust 2 15 C9 Dust or dust extract Eye/URTb 60 µL extract, 5–10 mg dust 10
7 dpe dust 2 15 C9 14
14 dpe dust 2 15 C9 21
Expt. 3 BLOOD INF A20 1 15 A20 Plasma + fresh whole bloodd Eye/intra coelemicc 60 µL plasma + 1 mL blood 20 & 24
Serva 1 15 Serva
Class 9 1 15 C9
Control Normal saline 2 16–17 - Normal saline Eye drop - 7
  1. C9, class 9; URT, upper respiratory tract.
  2. aDose for eye drop infection of donor birds only.
  3. bExtract administered by eye drop (n = 15), dust insufflated into nares (n = 5), laryngopharyngeal space (n = 5) or trachea (n = 5).
  4. cPlasma and whole fresh blood collected at 6 and 10 dpe of infected birds.
  5. dPlasma administered by eye drop and whole fresh blood by intra-coelemic (“abdominal”) injection to the same chicken.