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Figure 8 | Veterinary Research

Figure 8

From: Identification and functional characterization of a fish-specific tlr19 in common carp (Cyprinus carpio L.) that recruits TRIF as an adaptor and induces ifn expression during the immune response

Figure 8

Activation of ifn mediated by CcTlr19. A Luci-ifn and nf-kb reporter plasmids, rhRL-TK and CcTlr19-FUGW expression vector or an empty vector (control) were transfected into 293 T cells. After 48 h, Firefly and Renilla luciferase activities were detected, and the ratio was calculated. Means  ±  SD (n  =  4), *P  <  0.05, ***P  <  0.001, ****P  <  0.0001, t test. BD 293 T cells were transfected targeted plasmid. After 36 h, the cells were stimulated with poly(I:C), PGN and LPS for the indicated periods of time. Firefly and Renilla luciferase activities were detected, and the ratio was calculated. Means  ±  SD (n  =  4), *P  <  0.05, ***P  <  0.001, ****P  <  0.0001, two-way ANOVA.

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