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Figure 7 | Veterinary Research

Figure 7

From: Identification and functional characterization of a fish-specific tlr19 in common carp (Cyprinus carpio L.) that recruits TRIF as an adaptor and induces ifn expression during the immune response

Figure 7

Tlr19 colocalizes and interacts with TRIF. A HeLa cells transiently transfected with Tlr19-GFP and TRIF-mCherry-N1, MyD88-mCherry-N1 or TIRAP-mCherry-N1. After 24 h, the cells were fixed with 4% paraformaldehyde, stained with DAPI and subjected to confocal microscopy analysis. Green signals represent CcTlr19, red signals display adaptors, and blue staining indicates the nucleus. B 293 T cells were transfected with empty vector, Tlr19, TRIF or Tlr19  +  TRIF together with Luci-ifn and rhRL-TK reporter plasmids. After 48 h of transfection, all luciferase activities were calculated by normalization to Renilla luciferase activity, and the results are shown as the fold change compared to the control group (empty vector). Means  ±  SD (n  =  3), **P  <  0.01, ***P  <  0.001, one-way ANOVA.

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