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Figure 10 | Veterinary Research

Figure 10

From: Identification and functional characterization of a fish-specific tlr19 in common carp (Cyprinus carpio L.) that recruits TRIF as an adaptor and induces ifn expression during the immune response

Figure 10

CcTlr19 is modified with N-linked glycosylation. A N-X-S/T consensus sequence of CcTlr19. B EPC cells seeded in 6-well plates were transfected with the indicated plasmids (CcTlr19-GFP or an empty vector) separately. After 48 h, the whole-cell lysate was subjected to immunoblot assay with anti-GFP and anti-β-actin Abs. C Glycosidase digested CcTlr19. EPC cells seeded in 6-well plates were transfected with CcTlr19 or an empty vector, and the whole-cell lysate was digested with PNGase F or not (control). D A Luci-ifn-1 plasmid, rhRL-TK, together with CcTlr19 expression vector or an empty vector (control) were transfected into 293 T cells. After 48 h, Firefly and Renilla luciferase activity was detected, and the ratio was calculated. Means  ±  SD (n  =  4), *P  <  0.05, **P  <  0.01, ***P  <  0.001, ****P  <  0.0001, one-way ANOVA.

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Veterinary Research

ISSN: 1297-9716