Table 1 Sequences of the primers used for RT-qPCR.
Primers name | Directiona | Sequence (5′ → 3′) |
|---|---|---|
PDCoV | F | ACCTGTTTCCTTCGCCTTGA |
R | ACAGCGTCTGGTTGAGTTCC | |
IL-1β | F | AACGTGCAGTCTATGGAGT |
R | GAACACCACTTCTCTCTTCA | |
IL-6 | F | CTGGCAGAAAACA ACCTGAACC |
R | TGATTCTCATCAAGCAGGTCTCC | |
IL-12 | F | CGTGCCTCGGGCAATTATA |
R | CGCAGGTGAGGTCGCTAGTT | |
TNF-α | F | AACCTCAGATAAGCCCGTCG |
R | ACCACCAGCTGGTTGTCTTT | |
IFN-α | F | TCTCATGCACCAGAGCCA |
R | CCTGGACCACAGAAGGGA | |
IFN-β | F | AGTGCATCCTCCAAATCGCT |
R | GCTCATGGAAAGAGCTGTGGT | |
β-actin | F | TGACTGACTACCTCATGAAGATCC |
R | TCTCCTTAATGTCACGCACGATT |