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Figure 2 | Veterinary Research

Figure 2

From: Antiviral effects of ergosterol peroxide in a pig model of porcine deltacoronavirus (PDCoV) infection involves modulation of apoptosis and tight junction in the small intestine

Figure 2

Antiviral effect of EP on PDCoV replication in piglets. A Effect of EP on fecal viral shedding. Fecal samples of the indicated groups were collected from 0 dpi (the day before PDCoV infection) to 4 dpi. The relative viral RNA level in the fecal samples was analyzed by RT-qPCR. B Effect of EP on virus distribution. Different tissue samples of the indicated groups were collected at 5 dpi. The relative viral RNA level in the collected samples was analyzed by RT-qPCR. C Western blot analysis of proteins from jejunal and ileal tissues probed with the anti-PDCoV N antibody. The loading was normalized with β-actin. D Fluorescence imaging of jejunal and ileal tissues. Red fluorescence represents the PDCoV distribution, and blue fluorescence represents the nuclear distribution. Scale bars, 20 μm. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.

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Veterinary Research

ISSN: 1297-9716