Figure 4

Linear B cell epitope map of full-length S protein of indicated PEDVs. A Diagram depicting the main features of the PEDV S protein, including: putative cleavage site between S1 and S2 subunits at aa 726, signal peptide (aa 1–18), Domain 0 (aa 19–230), and the transmembrane domain (TM, aa 1325–1347). Functionally conserved S1 domain is at aa 231–733, whereas the S2 domain at aa 741–1344. Four subdomains are found in S1 domain, which include S1-NTD (S1 N-terminal domain, aa 231–471), S1-CTD (S1 C-terminal domain, aa 504–637), and Subdomains 1 and 2 (SD-1 and SD-2, aa 638–733). The numbers indicate the amino acid position relative to the S protein of virulent CV777 (GenBank ID: AF353511.1). The blue blocks represent the relative positions of protein regions where the neutralizing epitopes have been experimentally verified. B The linear B cell epitope map was obtained by using BepiPred-2.0 server. The amino acid residues with scores above the threshold value that was set at 0.5 were predicted to be part of an epitope and colored in yellow on the graph. Y-axes depicts residue scores and X-axes indicates amino acid positions in the sequence, which is also relative to the diagram on the top (A).