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Figure 7 | Veterinary Research

Figure 7

From: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation

Figure 7

Electron microscopic visualization of the degradation of Matrigel reconstituted basement membrane. The rGAPDH-harboring polystyrene beads (A, B) or the BSA-harboring beads (C, D) were incubated with plasminogen and tPA, and then added on the 3-μm filters in Transwell cell culture chamber inserts previously coated with Matrigel reconstituted basement membrane. After an incubation of 40 h, the filters were fixed with 2.5% glutaraldehyde, and examined in scanning electron microscope. B and D are the enlarged view of a part of A and C, respectively.

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