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Figure 6 | Veterinary Research

Figure 6

From: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation

Figure 6

Binding activity of the rGAPDH protein to plasminogen and the activation of the rGAPDH-bound plasminogen to plasmin. A ELISA was performed to characterize the ability of immobilized rGAPDH binding plasminogen. B Far Western blot analysis of binding activity of rGAPDH to plasminogen. Bound plasminogen was determined by anti-plasminogen antibody. BSA was chosen as negative control for non-specific binding. C Plasminogen bound to the coated rGAPDH in microtiters plate was activated to plasmin by tPA. The presence of lysine analogue, ε-ACA decreased the binding of plasminogen to rGAPDH. Activity of plasmin was evaluated by adding chromogenic substrate, and OD405nm was measured. Data are expressed as mean ± SD of at least three experiments with samples in triplicate. **P < 0.01.

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Veterinary Research

ISSN: 1297-9716