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Figure 4 | Veterinary Research

Figure 4

From: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation

Figure 4

Microtiter plate adhesion assay (MPAA) for the binding of the rGAPDH protein to cell membrane proteins. Microtiter plates were coated with membrane protein of PK-15 or NCI-H292 cells. Increasing concentrations of rGAPDH protein were added to individual wells (A: PK-15; C: NCI-H292). Bound rGAPDH was detected with anti-His-tag monoclonal antibody compared with the wells with no added protein. The adhesion of 50 μg/mL of rGAPDH to the cells (B: PK-15; D: NCI-H292) was inhibited by anti-rGAPDH serum but not by the preimmune serum. Data are expressed as mean ± SD of at least three experiments with samples in triplicate. ** indicate P < 0.01.

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Veterinary Research

ISSN: 1297-9716