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Figure 1 | Veterinary Research

Figure 1

From: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation

Figure 1

Expression and purification of the rGAPDH. A SDS-PAGE analysis of the rGAPDH. B Western blotting with anti-His-tag monoclonal antibody. M, protein molecular weight marker, lane 1 and 2, whole cell lysate of E. coli BL21 carrying recombinant vector pET-28a-gapdh before and after induction by IPTG overnight; lane 3, purified rGAPDH through Ni-chelating affinity chromatography.

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