Impacts of increasing challenge with Eimeria maxima on the growth performance and gene expression of biomarkers associated with intestinal integrity and nutrient transporters

Table 1 List of primers used for qPCR

Gene symbol	Accession Number	Forward primer	Reverse primer
GAPDH^a	NM_204305.1	CCTCTCTGGCAAAGTCCAAG	GGTCACGCTCCTGGAAGATA
CLDN1^b	NM_001013611.2	TGGAGGATGACCAGGTGAAGA	CGAGCCACTCTGTTGCCATA
CLDN2^b	NM_001277622.1	CCTGCTCACCCTCATTGGAG	GCTGAACTCACTCTTGGGCT
OCLDN^b	NM_205128.1	ACGGCAGCACCTACCTCAA	GGCGAAGAAGCAGATGAG
ZO1^b	XM_015278981.2	CAACTGGTGTGGGTTTCTGAA	TCACTACCAGGAGCTGAGAGGTAA
ZO2^b	NM_204918.1	ATCCAAGAAGGCACCTCAGC	CATCCTCCCGAACAATGC
JAM2^b	NM_001006257.1	AGCCTCAAATGGGATTGGATT	CATCAACTTGCATTCGCTTCA
MUC2^c	JX284122.1	ATGCGATGTTAACACAGGACTC	GTGGAGCACAGCAGACTTTG
GLUT5 (SLC2A5)^d	XM_417596.6	TTGCTGGCTTTGGGTTGTG	GGAGGTTGAGGGCCAAAGTC
SGLT1 (SLC5A1)^d	NM_001293240.1	GCCGTGGCCAGGGCTTA	CAATAACCTGATCTGTGCACCAGT
B⁰AT (SLC6A19)^d	XM_419056.6	GGGTTTTGTGTTGGCTTAGGAA	TCCATGGCTCTGGCAGAGAT
B⁰⁺AT (SLC7A9)^d	NM_001199133.1	CAGTAGTGAATTCTCTGAGTGTGAAGCT	GCAATGATTGCCACAACTACCA
EAAT (SLC1A1)^d	XM_424930.6	TGCTGCTTTGGATTCCAGTGT	AGCAATGACTGTAGTGCAGAAGTAATATATG
GLUT2 (SLC2A2)^d	NM_207178.1	TCATTGTAGCTGAGCTGTT	CGAAGACAACGAACACATAC
CAT1 (SLC7A1)^d	XM_015277945.2	CCAAGCACGCTGATAAAG	TACTCACAATAGGAAGAAGGG
LAT2 (SLC7A6)^d	XM_025154295.1	TCAGCTTCAGTTACTGGTT	GCACAACCACGAGAAATAC
GLUT1(SLC2A1)^d	NM_205209.1	CTTTGTCAACCGCTTTGG	TGTGCCCCGGAGCTTCT
LAT1 (SLC7A5)^d	NM_001030579.2	GATTGCAACGGGTGATGTGA	CCCCACACCCACTTTTGTTT

GAPDH glyceraldehyde-3-phosphate dehydrogenase, CLDN1 Claudin 1 [49], CLDN2 Claudin 2 [50], OCLDN Occludin [51], ZO1 Zonula occludens 1 [52], ZO2 Zonula occludens 2 [53], JAM2 Junctional adhesion molecule 2 [48], MUC2 Mucin [47], GLUT5 Glucose transporter 5, SGLT1 sodium glucose transporter 1, B⁰AT Na⁺-dependent amino acid transporter, B⁰⁺AT Na⁺-independent amino acid transporter, EAAT Excitatory amino acid transporter, GLUT2 Glucose transporter 2, CAT1 Cationic amino acid transporter, LAT2 Na⁺-dependent neutral/cationic amino acid transporter, GLUT1 Glucose transporter 1, LAT1 L-type amino acid transporter 1.
^aHousekeeping gene.
^bTight junction proteins.
^cMucin.
^dNutrient transporters [24].

ISSN: 1297-9716