Bacteria clearance assay in vivo. V. parahaemolyticus (A) or S. aureus (B) was injected into normal or MnHippo-dsRNA/GFP-dsRNA-injected prawns. The same volume of haemolymph (500 μL) was extracted from prawns at 20 min after injection in different groups, diluted with sterile PBS, and cultured on LB medium plates at 37 °C overnight. The number of bacterial colonies was counted. The data are shown as the mean values ± S.D. (N = 10). The asterisks indicate significant differences (** P < 0.01).