Chimeric HP-PRRSV2 containing an ORF2-6 consensus sequence induces antibodies with broadly neutralizing activity and confers cross protection against virulent NADC30-like isolate

Table 1 PCR primers used for the construction of PRRSV infectious clones.

No	Primer	Sequence (5ʹ-3ʹ)^a	Length (bp)
1	JSTZ1712-12-PacI-F1	AGCTCGTTAATTAATACATGACGTATAGGTGTTGGCT	37
2	JSTZ1712-12-AflII-R1	CATAGGTGCTTAAGTTCATTACCACCTGTAACGGAT	36
3	JSTZ1712-12-AflII-F2	ATCCGTTACAGGTGGTAATGAACTTAAGCACCTATG	36
4	JSTZ1712-12-AscI-R2	CCTTTCTGGCGCGCCCGAAAC	21
5	JSTZ1712-12-AscI-F3	GTTTCGGGCGCGCCAGAAAGG	21
6	JSTZ1712-12-1R3	*AGCGAGGAGGCTGGGACCAT*GCCGGCCTTTTTTTTTTTTTTTTTTTTTAATTACGGCCGCATGGTTCT	68
7	JSTZ1712-12-NotI-2R3	ACAGGCGGCCGCGTCCCATTCGCCATTACCGAGGGGACGGTCCCCTCGGAATGTTGCCCAGCCGGCGCCAGCGAGGAGGCTGGGACCAT^b	89
8	PRRSV2-AscI-ORF2-CON-F3	TTTCGGGCGCGCCAGAAAGGGAAAATTTATAAAGCTAATGCCACCAGCATGAGGTTTCATTTTCCCCCGGGCCCT	75
9	PRRSV2-ORF6-CON-R	TTCTTTTTCTTTTGCTGCTTGCCGTTGTTATTTGGCATATTTGACAAGGTTTACCACTCCCTGT	64
10	PRRSV2-ORF6-CON-F2	ACAGGGAGTGGTAAACCTTGTCAAATATGCCAAATAACAACGGCAAGCAGCAAAAGAAAAAGAA	64

^aThe unique restriction enzyme sites used for cloning purposes are underlined.
^bThe hepatitis D virus ribozyme sequence is shown in italic and the overlapped region is highlighted in bold.

ISSN: 1297-9716