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Figure 6 | Veterinary Research

Figure 6

From: Chimeric HP-PRRSV2 containing an ORF2-6 consensus sequence induces antibodies with broadly neutralizing activity and confers cross protection against virulent NADC30-like isolate

Figure 6

Frequencies of PRRSV-specific IFN-γ secreting cells. PBMC were isolated from each groups of pigs at 0 dpc (42 dpi), 7 dpc (49 dpi), 14 dpc (56 dpi) and stimulated with or without HP-PRRSV2 JXA1-R strain at 0.01 MOI for 20 h. The intracellular IFN-γ expression on total CD3+ T cells was further analyzed by flow cytometry. Representative dot-plots from each group were shown with frequencies of PRRSV-specific IFN-γ secreting cell population (left panel). The overall frequencies of PRRSV-specific IFN-γ secreting cells in each group were compared and are shown as bar graphs at the indicated time points (right panel). No significant difference was detected between rJS-ORF2-6-CON inoculated and TJM-F92 vaccinated pigs.

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Veterinary Research

ISSN: 1297-9716