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Figure 4 | Veterinary Research

Figure 4

From: Chimeric HP-PRRSV2 containing an ORF2-6 consensus sequence induces antibodies with broadly neutralizing activity and confers cross protection against virulent NADC30-like isolate

Figure 4

Dynamics of rectal temperature, weight gain, virus load, antibody and IFN-γ levels during inoculation and challenge studies. After the challenge of NADC30-like SD17-38 isolate, all positive control pigs and two of TJM-F92 vaccinated pigs showed high fever. However, the rectal temperature of rJS-ORF2-6-CON inoculated and mock-infected pigs did not reach 40 °C during the whole experiment (A). rJS-ORF2-6-CON inoculated pigs had significantly higher weight gain than TJM-F92 vaccinated pigs at 14 dpc (B). Dynamics of viremia were detected by real-time RT-PCR assay [32]. Compared with TJM-F92 vaccinated pigs, the virus load was significantly lower in rJS-ORF2-6-CON inoculated pigs since 7 dpc (C). PRRSV-specific antibody level was detected by IDEXX HerdCheck*PRRS×3 Antibody Detection ELISA Kit. The threshold for seroconversion was set at a sample-to-positive (s/p) ratio of 0.4. PRRSV-specific antibody could be detected in all PRRSV-infected pigs from 14 dpi to the end of the study (D). IFN-γ level was detected using a commercial Porcine IFN-gamma ELISA kit. No significant difference of IFN-γ amounts was detected in rJS-ORF2-6-CON inoculated and TJM-F92 vaccinated pigs at 42 dpi and 14 dpc (E). Each bar represents the average for all pigs in each group ± standard deviation (SD). The significant difference is marked with an asterisk.

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