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Figure 2 | Veterinary Research

Figure 2

From: Chimeric HP-PRRSV2 containing an ORF2-6 consensus sequence induces antibodies with broadly neutralizing activity and confers cross protection against virulent NADC30-like isolate

Figure 2

Strategy to construct the full-length cDNA clones of avirulent HP-PRRSV2 JSTZ1712-12 isolate and a chimeric virus containing ORF2-6-CON. The strategy was adopted from our previous study [25]. The pACYC177-CMV-Stuffer fragment including the unique restriction enzymes was shown in the upper part (A). The three overlapped fragments of the rJSTZ1712-12 (B) and rJS-ORF2-6-CON (C) genomes were produced by PCR amplification and are shown in the bottom part.

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Veterinary Research

ISSN: 1297-9716