Generation and evaluation of IgY-scFv based mimetics against canine parvovirus

Table 1 Comparison of IgY-scFv and IgY-peptide mimetic detection on clinical samples.

Sample	IgY-scFv (Mean ± SD)	IgY-peptide (Mean ± SD)	ICA	PCR	Sample	IgY-scFv (Mean ± SD)	IgY-peptide (Mean ± SD)	ICA	PCR
1	0.52 ± 0.02	0.32 ± 0.00	+	+	16	0.51 ± 0.01	0.48 ± 0.12	+	+
2	0.52 ± 0.01	0.46 ± 0.03	+	+	17	0.85 ± 0.03	0.99 ± 0.17	+	+
3	0.59 ± 0.01	0.52 ± 0.02	+	+	18	0.67 ± 0.01	0.59 ± 0.13	+	+
4	0.14 ± 0.03	0.05 ± 0.00	−	−	19	0.71 ± 0.01	0.54 ± 0.16	+	+
5	1.51 ± 0.00	0.35 ± 0.04	+	+	20	0.84 ± 0.05	0.31 ± 0.05	+	+
6	0.11 ± 0.02	0.03 ± 0.01	−	−	21	0.67 ± 0.01	0.32 ± 0.04	+	+
7	1.99 ± 0.06	1.56 ± 0.35	+	+	22	2.2 ± 0.13	1.43 ± 0.27	+	+
8	0.56 ± 0.03	0.64 ± 0.02	+	+	23	0.87 ± 0.01	0.59 ± 0.18	+	+
9	0.82 ± 0.00	0.66 ± 0.03	+	+	24	0.45 ± 0.01	0.32 ± 0.02	+	+
10	1.1 ± 0.05	0.96 ± 0.03	+	+	25	0.58 ± 0.00	0.93 ± 0.03	+	+
11	1.47 ± 0.08	0.74 ± 0.04	+	+	26	0.15 ± 0.02	0.04 ± 0.00	−	−
12	0.14 ± 0.03	0.06 ± 0.01	−	−	27	0.33 ± 0.00	0.51 ± 0.19	+	+
13	0.83 ± 0.03	0.77 ± 0.03	+	+	28	1.98 ± 0.01	0.96 ± 0.03	+	+
14	0.86 ± 0.01	0.57 ± 0.05	+	+	NC	0.03 ± 0.02	0.07 ± 0.01
15	0.84 ± 0.04	0.22 ± 0.04	+	+

“+” the ICA positive samples, “−” the ICA negative samples; NC, negative control, the sample was replaced by BSA. Statistical principle, P/N > 2.0 (P samples value; N NC value), it can be judged as positive at the level of 99.9%. Results are means of three replicates. The PCR and ICA results were in accordance to [4].

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