Figure 1

The design and synthesis of IgY-peptide. A Obtained anti-CPV IgY-scFv screened by T7 phage display technology which had confirmed specificity and neutralization ability; A total of 13 epitopes were predicated on scFv sequence by PREDICTED ANTIGENIC PEPTIDES software (UCM, Madrid, Spain). B The three-dimensional protein structure model was obtained by I-TASSER software (a hierarchical approach to protein structure and function prediction; UMich, Ann Arbor, MI, USA). C Solid phase peptide synthesis scheme. IgY-peptide was synthetized using solid phase Fmoc on a PEG-Polystyrene support resin. Upon synthesis completion, side chain protecting groups were removed and the peptide was simultaneously cleaved from the resin. The cleaved and deprotected peptide material was further precipitated, washed and dissolved in a buffer containing H₂O/ACN/HOAC prior to lyophilization. D The determination of IgY-peptide by HPLC. Retention time of mimetic peptide: 11.855 min; Analytical column type: SHIMADZU Inertsil ODS-SP (4.6 × 250 mm × 5 µm); Pump A: 0.1% trifluoroacetic in 100% water, Pump B: 0.1% trifluoroacetic in 100% acetonitrile; Wavelength: 220 nm. E MS analysis of purified mimetic peptide. ESI_MS. value = (M + n*H)n, M is the correct mass, n is an integer and n > 0, while n is range.