Perspectives for improvement of Mycoplasma hyopneumoniae vaccines in pigs

Maes, Dominiek; Boyen, Filip; Devriendt, Bert; Kuhnert, Peter; Summerfield, Artur; Haesebrouck, Freddy

doi:10.1186/s13567-021-00941-x

Veterinary Research

Table 5 Efficacy and/or immune responses of experimental vaccines (subunit, membrane proteins, culture supernate) against Mycoplasma hyopneumoniae tested in pigs.

From: Perspectives for improvement of Mycoplasma hyopneumoniae vaccines in pigs

Antigen	Vaccine type	Adjuvant/carrier	Route	Nb of vaccinations	Challenge infection	Decrease of			Humoral response		CMI response^b	Other/comments	References
Antigen	Vaccine type	Adjuvant/carrier	Route	Nb of vaccinations	Challenge infection	Lung lesions	Clinical signs	M. hyopneumoniae numbers	Serum	BALF ^c	CMI response^b	Other/comments	References
P97	Subunit	Complete Freund’s adjuvant	IM	2	Yes	No	No		Yes^d				[98]
J strain	Membrane proteins	Five different adjuvants^e	IM-IP	2	Yes	Yes^e			IgG, IgA	IgA, IgG		No effect on ADG; Humoral response in BALF: only after challenge, greater in IM groups	[48]
Strain 1986–1	Cell-free culture supernate	Al(OH)3	IM	2	Yes	Yes		Yes	IgG (low)				[99]
Strain 1986–1	Cell-free culture supernate	Al(OH)3	IM	2	Yes	Yes		Yes		IgA, IgG		Less macrophages, lymphocytes and TNFα in lungs Humoral response in BALF only after challenge	[100]
P97 (RR1) ^a	Chimeric subunit with Pseudomonas exotoxin A		SC (mice)-IM (pigs)	2	No				IgG				[101]
P97 (R1R2)	Chimeric subunit with N-terminal region A. pleuropneumoniae ApxIII	Freund’s adjuvant (mice), Al(OH)3 (pigs)	SC (mice)-IM (pigs)	2	Yes (pigs)	Yes	Yes		IgG1, IgG2a (mice)		Higher IFN-γ and IL-4 (mice)	Also protection against A. pleuropneumoniae	[102]
P97, P42, NrdF	Chimeric subunit	LTB^f	IM-IN	2	Yes	No	No	No	IgG	IgA		IgA in BALF only after challenge Commercial vaccine: lower serological response, better protection	[21]
P102 and 8 fragments of P97 /P102 paralogs	Subunit	Al(OH)3 and polymer based (Montanide™)	IM	3	Yes	No	No	No	IgG	No IgA		Less cilia damage, less Il-1, Il-6, TNFα in BALF Commercial vaccine: lower serological response, better protection	[52]

IM, Intramuscular; SC, Subcutaneous; IN, Intranasal; IP, intraperitoneal.
^aStudy was done in pigs and mice.
^bCMI response was tested by stimulation of splenocytes.
^cBALF bronchoalveolar lavage fluid.
^dtested by Western blotting for reactivity with whole cell lysates.
^eThe membrane preparations were formulated with one of the following adjuvants: 1) Auspharm oil, 2) Alhydrogel, 3) Algammulin, 4) DEAE dextran with Auspharm oil, 5) DEAE dextran with mineral oil. Lung lesions were significantly reduced with all formulations compared to non-vaccinated pigs, but there were no significant differences between the formulations.
^fLTB B subunit of heat-labile enterotoxin of E. coli.

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ISSN: 1297-9716

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