Table 3 Experimental DNA vaccines against Mycoplasma hyopneumoniae tested in mice.
From: Perspectives for improvement of Mycoplasma hyopneumoniae vaccines in pigs
Antigen | Vaccine type | Vector/adjuvant | Route | Nb of vaccinations | Humoral response | CMI responsea | Other/comments | References |
|---|---|---|---|---|---|---|---|---|
Serum | ||||||||
P42 | DNA | pcDNA3 | IM | 2 | IgG, IgG1, IgG2a, IgG2b, IgG3 IgG2a/IgG1 = 1.1) | IFN-γ, Il-2, Il-4 | Serum inhibited growth of M. hyopneumoniae | [87] |
P71 | DNA | Mycobacterium tuberculosis ESAT-6 gene sequences | IM | 2 | IgG1, IgG2a (higher responses and IgG2a/IgG1 ratio in group with adjuvant) | IFN-γ (higher in group with adjuvant), no Il-10 |  | [88] |
P36, P46, NrdF, and P97or P97R1 | Subunit (cocktail), DNA, combination | Subunit: Aluminium pcDNA3 | Subunit: SC DNA: IM | 1 | Subunit, combination: IgG against each antigen DNA: IgG only against P46 | IFN-γ | Commercial vaccine: no anti-P97 antibodies | [89] |
P37, P42, P46, P95 | Subunit (cocktail) and DNA | Subunit: Aluminium pcDNA3 | IM | 2 |  | IFN-γ, lower TNFα and Il-1 | Strongest response for P42 and P95 (subunit) and for P46 (DNA) | [90] |
P46, HSP70, MnuA antigens | Subunit (cocktail) and DNA | Subunit: Freund’s adjuvant pcDNA3.1 | Subunits: IP DNA: IM | 3 | IgG | IFN-γ, Il-10, no Il-4 | Mixed response, but predominantly Th1 | [91] |