Quantification of IS900 sequence by qPCR in CaMØs and supernatants from non-vaccinated and vaccinated goats. Quantification was performed after 24 h of incubation with a MOI of 10:1 of Mycobacterium avium subsp. Paratuberculosis (Map) (10 bacilli/macrophage). Amplification slopes ranged between −3.48 and −3.59 with a correlation coefficient (R2) of 0.992–0.997 and an efficiency of 90.85−93.87%. Data is represented as picograms in bar plots as mean values and standard deviations (n = 6).