Figure 5

Over-expression of SYNCRIP leads to the reduction of NS1 mRNA and protein expression. A Western blotting analysis of SYNCRIP protein levels in PK-15 cells with SYNCRIP over-expression using anti-His monoclonal antibodies. PK-15 as the cells without transfection, PK^V as the cells transfected with blank vector, PK^SYNCRIP as the cells transfected with the vector expressing His-SYNCRIP. B Western blotting analysis of SYNCRIP protein levels in PK-15 cells with SYNCRIP over-expression using SYNCRIP monoclonal antibodies. C Cell viability of cell lines stably overexpressing SYNCRIP. D Western blotting analysis of viral NS1, NS2, VP1 and VP2 proteins expression levels in PK-15 cells with SYNCRIP over-expression. E Western blotting analysis of NS1 protein expression levels in PK-15 cells of SYNCRIP over-expression. F Western blotting analysis of NS1 protein levels in PK-15 cells co-transfected with different doses of SYNCRIP (0, 1, 2, 4 or 8 μg) for 24 h.G Western blotting analysis of NS1 protein levels in ST cells co-transfected with different doses of SYNCRIP (0, 1, 2, 4 or 8 μg) for 24 h. H Q-PCR analysis of NS1 mRNA levels in PK-15 cells co-transfected with different doses of SYNCRIP (0, 1, 2, 4 or 8 μg) for 24 h. I Q-PCR analysis of NS1 mRNA levels in ST cells co-transfected with different doses of SYNCRIP (0, 1, 2, 4 or 8 μg) for 24 h. The results are shown as the mean ± SD (n = 3). *p < 0.05 versus untreated cells with the vector pCDNA-His-SYNCRIP over-expression.