Figure 4

Knockout of SYNCRIP inhibits the viral replication in PPV‑infected cells. A Schematic chromatogram representation of sgRNA targeting at the SYNCRIP genomic region. PAM sequences are underlined and highlighted in green. sgRNA targeting sites are underlined and highlighted in red. Red arrows indicate gRNA targeting sites. B Western blotting analysis of the SYNCRIP expression in PK-15 cells infected with CRISPR/Cas9 lentivirus and then selected by puromycin. The lentiviruses contain gRNA-63 and gRNA-210. C Cell viability of cell lines stably knockout for SYNCRIP. D-E Knockout of SYNCRIP inhibits PPV progeny virion production. PK-15, PK^SYNCRIP+/+ and PK^{SYNCRIP−/−} cells were infected with PPV (MOI = 1) for 24 h. The relative fold-change PPV DNA copies and viral titers were determined by q-PCR and TCID₅₀ assay. The results are shown as the mean ± SD (n = 3). *p < 0.05 versus PK-15 cells with the same treatment.