Figure 3

PPV infection up-regulates SYNCRIP expression in vitro and in vivo. PK-15 cells were infected with 1 MOI of PPV or mock infection for the time indicated, the mRNA level of SYNCRIP (A), the protein level of SYNCRIP (B) and the relative viral titers of different infection times were measured by TCID₅₀ (C). D-E qPCR and western blotting analysis of SYNCRIP mRNA and protein levels in PK-15 cells infected with different doses of PPV (MOI = 0, 0.5, 1, or 2) for 24 h. β-actin served as an internal control. F–G Western blotting and qPCR analysis of SYNCRIP protein and mRNA levels in different tissues of PPV infected pregnant sows. H–I Western blotting and q-PCR analysis of viral VP2 protein levels and DNA copies in different tissues of PPV infected pregnant sows. β-actin served as an internal control. The results are shown as the mean ± SD (n = 3). *p < 0.05, versus mock infected cells at same time points. ^#p < 0.05, versus mock tissues at same tissue.