Inhibiting the Cxcl1–Cxcr2 axis in the GSDMD−/− mice improves host defence during S. aureus skin infection. For one group of GSDMD−/− mice, 100 μL of 100 μg SB225002 was injected i.p. 1 h prior to infection and 23 h post-infection with 1 × 107 CFU S. aureus, and abscess tissue was excised on day 2 post-infection. A The lesion size from the dorsal area of mice from each group was measured. B Representative pictures are shown. C Bacterial burden in the skin was assessed. D Skin tissue structures were observed by H&E (magnification of 200 ×). E Representative immunohistochemical staining of Gr-1 was performed in the skin sections. F The homogenate supernatants of skins were also used to determine the activity of MPO. E: epidermis; D: dermis; A: adipose tissue; M: muscle fibres. All data are shown as the mean ± SEM. n = 7–8 per group. Data pooled from 2 independent experiments. One-way ANOVA with Tukey–Kramer post hoc tests was performed. Statistical significance is indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.