GSDMD inhibits Cxcl1 secretion in a NF-κB-dependent manner upon S. aureus challenge. WT and GSDMD−/− BMDMs were untreated or exposed to S. aureus at an MOI of 1:50 for the indicated times. A Cxcl1 mRNA levels were measured at 6 h post-infection by qRT-PCR. B The cell lysates were examined for the expression levels of p-P65 by Western blotting. GAPDH served as a loading control. WT and GSDMD−/− mice were infected s.c. with 1 × 107 CFU S. aureus, and abscess tissue was excised on day 2 post-infection. C Skin tissue mRNA was examined for Cxcl1 by qRT-PCR. D Skin tissues were homogenized and then immunoblotted for p-P65, p-IκBα, IκBα, and GAPDH. Data A are shown as the mean ± SEM (three independent experiments) of each group (n = 3). Data C are shown as the mean ± SEM. n = 8 per group. Data were pooled from 2 independent experiments. Student’s t-test was performed. Statistical significance is indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.