GSDMD-deficient mice display increased susceptibility to S. aureus skin infection. WT and GSDMD−/− mice were infected s.c. with 1 × 107 CFU S. aureus. A The lesion size from the dorsal area of mice from each group was measured. B Representative pictures are shown on day 2 post-infection. C Bacterial burden in the skin was assessed. D Skin tissue structures were observed by H&E (magnification of 200 ×). E: epidermis; D: dermis; A: adipose tissue; M: muscle fibres. All data are shown as the mean ± SEM. n = 9 per group. Data pooled from 2 independent experiments. Student’s t-test was performed. Statistical significance is indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.