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Table 6 Upregulation of the spa gene activity of Staphylococcus aureus by three different culture preparations obtained from bovine non-aureus staphylococci (NAS) isolates from milk and teat apices (TA)

From: Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro

NAS species (no.)1 SP2 [no. (%)] SPK [no. (%)] CS [no. (%)] All culture preparations [no. (%)]
Milk (45) TA (14) Total (59) Milk (45) TA (14) Total (59) Milk (45) TA (14) Total (59) Milk (45) TA (14) Total (59)
All (59)
 Severe effect3 20 (44.4) 8 (57.1) 28 (47.4) 6 (13.4) 2 (14.3) 8 (13.6) 0 0 0 26 (19.2) 10 (23.8) 36 (20.3)
 Moderate effect 17 (37.8) 2 (14.3) 19 (32.2) 11 (24.4) 8 (57.1) 19 (32.2) 4 (8.9) 1 (7.1) 5 (8.5) 32 (23.7) 11 (26.2) 43 (24.3)
 Slight effect 1 (2.2) 0 1 (1.7) 20 (44.4) 0 20 (33.9) 18 (40.0) 7 (50.0) 25 (42.4) 39 (28.9) 7 (16.7) 46 (26.0)
 No effect 7 (15.6) 4 (28.6) 11 (18.7) 8 (17.8) 4 (28.6) 12 (20.3) 23 (51.1) 6 (42.9) 29 (49.1) 38 (28.2) 14 (33.3) 52 (29.4)
S. chromogenes (34)
 Severe effect 11 (39.3) 4 (66.8) 15 (44.1) 2 (7.1) 0 2 (5.9) 0 0 0 13 (15.5) 4 (22.2) 17 (16.7)
 Moderate effect 15 (53.6) 1 (16.6) 16 (47.1) 5 (17.9) 5 (83.4) 10 (29.4) 2 (7.1) 1 (16.6) 3 (8.8) 22 (26.2) 7 (38.9) 29 (28.4)
 Slight effect 1 (3.6) 0 1 (2.9) 20 (71.4) 0 20 (58.8) 10 (35.7) 3 (50.0) 13 (38.2) 31 (36.9) 3 (16.7) 34 (33.3)
 No effect 1 (3.6) 1 (16.6) 2 (5.9) 1 (3.6) 1 (16.6) 2 (5.9) 16 (57.2) 2 (33.4) 18 (53.0) 18 (21.4) 4 (22.2) 22 (21.6)
S. epidermidis (11)
 Severe effect 0 1 (25.0) 1 (9.1) 0 1 (25.0) 1 (9.1) 0 0 0 0 2 (16.7) 2 (6.0)
 Moderate effect 1 (14.3) 0 1 (9.1) 0 0 0 0 0 0 1 (4.7) 0 1 (3.0)
 Slight effect 0 0 0 0 0 0 3 (42.9) 1 (25.0) 4 (36.4) 3 (14.3) 1 (8.3) 4 (12.0)
 No effect 6 (85.7) 3 (75.0) 9 (81.8) 7 (100) 3 (75.0) 10 (90.9) 4 (57.1) 3 (75.0) 7 (63.6) 17 (81.0) 9 (75.0) 26 (79.0)
S. simulans (14)
 Severe effect 9 (90.0) 3 (75.0) 12 (85.7) 4 (40.0) 1 (25.0) 5 (35.7) 0 0 0 13 (43.3) 4 (33.4) 17 (40.5)
 Moderate effect 1 (10.0) 1 (25.0) 2 (14.3) 6 (60.0) 3 (75.0) 9 (64.3) 2 (20.0) 0 2 (14.3) 9 (30.0) 4 (33.4) 13 (31.0)
 Slight effect 0 0 0 0 0 0 5 (50.0) 3 (75.0) 8 (57.2) 5 (16.7) 3 (25.0) 8 (19.0)
 No effect 0 0 0 0 0 0 3 (30.0) 1 (25.0) 4 (28.6) 3 (10.0) 1 (8.2) 4 (9.5)
  1. 1Culture preparations = SP–Supernatant; SPK–Supernatant + Proteinase K; CS–Cell suspension.
  2. 2Number of isolates.
  3. 3Regulation effect was rated according to the size of inhibition zone around the well (measured in mm) [12] and was classified as exhibiting no effect (≤ 10 mm), a slight effect (11–15 mm), a moderate effect (16–20 mm), and a severe effect (≥ 25 mm) on gene expression.