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Table 5 Downregulation of the hla gene activity of Staphylococcus aureus by three different culture preparations obtained from bovine non-aureus staphylococci (NAS) isolates from milk and teat apices (TA)

From: Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro

NAS species (no.)1 SP2 [no. (%)] SPK [no. (%)] CS [no. (%)] All culture preparations [no. (%)]
Milk (45) TA (14) Total (59) Milk (45) TA (14) Total (59) Milk (45) TA (14) Total (59) Milk (45) TA (14) Total (59)
All (59)
 Severe effect3 26 (57.8) 9 (64.3) 35 (59.3) 7 (15.6) 3 (21.4) 10 (17.0) 0 0 0 33 (24.5) 12 (28.5) 45 (25.4)
 Moderate effect 11 (24.4) 1 (7.1) 12 (20.3) 27 (60.0) 7 (50.0) 34 (57.6) 2 (4.5) 0 2 (3.4) 40 (29.6) 8 (19.0) 48 (27.1)
 Slight effect 1 (2.2) 0 1 (1.7) 3 (6.6) 0 3 (5.1) 16 (35.5) 9 (64.3) 25 (42.4) 20 (14.8) 9 (21.4) 29 (16.4)
 No effect 7 (15.6) 4 (28.6) 11 (18.7) 8 (17.8) 4 (28.6) 12 (20.3) 27 (60.0) 5 (35.7) 32 (54.2) 42 (31.1) 13 (30.9) 55 (31.1)
S. chromogenes (34)
 Severe effect 16 (57.1) 4 (66.8) 20 (58.8) 3 (10.7) 1 (16.6) 4 (11.7) 0 0 0 19 (22.6) 5 (27.8) 24 (23.5)
 Moderate effect 11 (39.3) 1 (16.6) 12 (35.3) 21 (75.0) 4 (66.8) 25 (73.6) 1 (3.6) 0 1 (2.9) 33 (39.3) 5 (27.8) 38 (37.3)
 Slight effect 0 0 0 3 (10.7) 0 3 (8.8) 9 (32.1) 5 (83.4) 14 (41.2) 12 (14.3) 5 (27.8) 17 (16.7)
 No effect 1 (3.6) 1 (16.6) 2 (5.9) 1 (3.6) 1 (16.6) 2 (5.9) 18 (64.3) 1 (16.7) 19 (55.9) 20 (23.8) 3 (16.6) 23 (22.5)
S. epidermidis (11)
 Severe effect 0 1 (25.0) 1 (9.1) 0 1 (25.0) 1 (9.1) 0 0 0 0 2 (16.7) 2 (6.0)
 Moderate effect 0 0 0 0 0 0 1 (14.3) 0 1 (9.1) 1 (4.7) 0 1 (3.0)
 Slight effect 1 (14.3) 0 1 (9.1) 0 0 0 2 (28.6) 1 (25.0) 3 (27.3) 3 (14.3) 1 (8.3) 4 (12.0)
 No effect 6 (85.7) 3 (75.0) 9 (81.8) 7 (100) 3 (75.0) 10 (90.9) 4 (57.1) 3 (75.0) 7 (63.6) 17 (81.0) 9 (75.0) 26 (79.0)
S. simulans (14)
 Severe effect 10 (100) 4 (100) 14 (100) 4 (40.0) 1 (25.0) 5 (35.7) 0 0 0 14 (46.6) 5 (41.6) 19 (45.3)
 Moderate effect 0 0 0 6 (60.0) 3 (75.0) 9 (64.3) 0 0 0 6 (20.0) 3 (25.0) 9 (21.4)
 Slight effect 0 0 0 0 0 0 5 (50.0) 3 (75.0) 8 (57.2) 5 (16.7) 3 (25.0) 8 (19.0)
 No effect 0 0 0 0 0 0 5 (50.0) 1 (25.0) 6 (42.8) 5 (16.7) 1 (8.3) 6 (14.3)
  1. 1 Number of isolates.
  2. 2Culture preparations = SP–Supernatant; SPK–Supernatant + Proteinase K; CS–Cell suspension.
  3. 3Regulation effect was rated according to the size of inhibition zone around the well (measured in mm) [12] and was classified as exhibiting no effect (≤ 10 mm), a slight effect (11–15 mm), a moderate effect (16–20 mm), and a severe effect (≥ 25 mm) on gene expression.