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Table 4 Downregulation of the rnaIII gene activity of Staphylococcus aureus by three different culture preparations obtained from bovine non-aureus staphylococci (NAS) isolates from milk and teat apices (TA) [no. (%)]

From: Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro

NAS species (no.)1

SP2 [no. (%)]

SPK [no. (%)]

CS [no. (%)]

All culture preparations [no. (%)]

Milk (45)

TA (14)

Total (59)

Milk (45)

TA (14)

Total (59)

Milk (45)

TA (14)

Total (59)

Milk (45)

TA (14)

Total (59)

All (59)

 Severe effect3

12 (26.7)

6 (42.8)

18 (30.5)

5 (11.1)

2 (14.3)

7 (11.9)

1 (2.2)

1 (7.1)

2 (3.4)

18 (13.4)

9 (21.4)

27 (15.2)

 Moderate effect

24 (53.3)

2 (14.3)

26 (44.1)

26 (57.8)

7 (50.0)

33 (56.0)

2 (4.5)

4 (28.6)

6 (10.2)

52 (38.5)

13 (30.9)

65 (36.8)

 Slight effect

1 (2.2)

2 (14.3)

3 (5.1)

3 (6.7)

1 (7.1)

4 (6.7)

17 (37.8)

4 (28.6)

21 (35.6)

21 (15.5)

7 (16.8)

28 (15.8)

 No effect

8 (17.8)

4 (28.6)

12 (20.3)

11 (24.4)

4 (28.6)

15 (25.4)

25 (42.4)

5 (35.8)

30 (50.8)

44 (32.6)

13 (30.9)

57 (32.2)

S. chromogenes (34)

 Severe effect

2 (7.1)

2 (33.4)

4 (11.7)

2 (7.1)

0

2 (5.9)

1 (3.6)

1 (16.6)

2 (5.9)

5 (6.0)

3 (16.7)

8 (7.8)

 Moderate effect

24 (85.7)

2 (33.4)

26 (76.5)

19 (67.9)

5 (83.4)

24 (70.6)

1 (3.6)

3 (50.0)

4 (11.7)

44 (52.4)

10 (55.5)

54 (53.0)

 Slight effect

1 (3.6)

1 (16.6)

2 (5.9)

3 (10.7)

0

3 (8.8)

13 (46.4)

1 (16.7)

14 (41.2)

17 (20.2)

2 (11.1)

19 (18.6)

 No effect

1 (3.6)

1 (16.6)

2 (5.9)

4 (14.3)

1 (16.6)

5 (14.7)

13 (46.4)

1 (16.7)

14 (41.2)

18 (21.4)

3 (16.7)

21 (20.6)

S. epidermidis (11)

 Severe effect

0

1 (25.0)

1 (9.1)

0

0

0

0

0

0

0

1 (8.3)

1 (3.0)

 Moderate effect

0

0

0

0

1 (25.0)

1 (9.1)

1 (14.3)

1 (25.0)

2 (18.2)

1 (4.7)

2 (16.7)

3 (9.0)

 Slight effect

0

0

0

0

0

0

0

0

0

0

0

0

 No effect

7 (100)

3 (75.0)

10 (90.9)

7 (100)

3 (75.0)

10 (90.9)

6 (85.7)

3 (75.0)

9 (81.8)

20 (95.3)

9 (75.0)

29 (88.0)

S. simulans (14)

 Severe effect

10 (100)

3 (75.0)

13 (92.8)

3 (30.0)

2 (50.0)

5 (35.7)

0

0

0

13 (43.4)

5 (41.6)

18 (42.9)

 Moderate effect

0

0

0

7 (70.0)

2 (50.0)

9 (64.3)

0

0

0

7 (23.3)

2 (16.7)

9 (21.4)

 Slight effect

0

1 (25.0)

1 (7.2)

0

0

0

4 (40.0)

3 (75.0)

7 (50.0)

4 (13.3)

4 (33.4)

8 (19.0)

 No effect

0

0

0

0

0

0

6 (60.0)

1 (25.0)

7 (50.0)

6 (20.0)

1 (8.3)

7 (16.7)

  1. 1 Culture preparations = SP–Supernatant; SPK–Supernatant + Proteinase K; CS–Cell suspension.
  2. 2 Number of isolates.
  3. 3 Regulation effect was rated according to the size of inhibition zone around the well (measured in mm) [12] and was classified as exhibiting no effect (≤ 10 mm), a slight effect (11–15 mm), a moderate effect (16–20 mm), and a severe effect (≥ 25 mm) on gene expression.