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Table 2 Final linear mixed effect model for the β-galactosidase liquid assay for rnaIII gene expression including the positive and negative controls

From: Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro

Predictor variables β1 SE2 LSM3 P value4
Intercept 2.24 0.20  
Time of measurement     0.0453
 1 h Referent 40.95  
 2 h −0.08 0.13 46.83  
 3 h 0.09 0.16 53.35  
 4 h 0.20 0.19 58.95  
Staphylococcal species5     < 0.0001
 S. aureus (negative control) Referent 196.52  
 S. aureus + S. schleiferi (positive control) −0.78 0.28 23.72  
 S. aureus + S. chromogenes −0.76 0.20 36.20  
 S. aureus + S. epidermidis −0.78 0.21 60.63  
 S. aureus + S. simulans −0.82 0.21 29.22  
Staphylococcal species x Time of measurement6     < 0.0001
  1. 1 Estimate.
  2. 2 Standard error.
  3. 3 Least square means after back transformation.
  4. 4 Overall P value for fixed effects.
  5. 5 Staphylococcus aureus rnaIII::lacZ reporter strain SH101F7 was used as negative control when growing alone and as positive control when in co-culture with Staphylococcus schleiferi strain 2898.
  6. 6 The interaction term is visualized in Figure 2A.