Figure 2

Effect of sodium selenite in the viability of SHK-1 cells infected with P. salmonis. A P. salmonis containing vacuoles (PCVs) per total cells in SHK-1 sodium selenite treated/untreated and infected/uninfected cells at 10 days post-infection (dpi). B Relative P. salmonis load (arbitrary units, AU) (left axis) represented by blue circles and cell viability percentage (right axis) in grey bars in SHK-1 sodium selenite treated/untreated and infected/uninfected cells. C Representative immunofluorescence microphotographs of SHK-1 monolayer exposed (Se +) or not (Se −) to sodium selenite (1 μM). Upper panels show uninfected cells, and lower panels show P. salmonis infected cells at 10 dpi with sodium selenite treatment. Bar = 10 μm. For B and C, data represent mean ± SD of 10 observations measured in at least two independent experiments (n = 10). One-way ANOVA and Tukey multiple comparisons between all treatments were performed. Different letters represent significant differences (p-value < 0.05; capital letters for left axis and lowercase letters for right axis comparisons).