Table 1 Primer sequences used for qRT-PCR analysis
From: Long non-coding RNA LNC_000641 regulates pseudorabies virus replication
Primer | Sequence (5′→3′) | Product |
|---|---|---|
IFN-alpha-Fwd | TCCAGAAACCTGCAAGACAG | IFN-alpha |
IFN-alpha-Rev | ATGGGCTTGTTAGTCTGTGAG | |
IFN-beta-Fwd | ACCACAGCTCTTTCCATGAG | IFN-beta |
IFN-beta-Rev | CAGGGACCTCAAAGTTCATCC | |
IFN-gamma-Fwd | AATGGTAGCTCTGGGAAACTG | IFN-gamma |
IFN-gamma-Rev | ACTTCTCTTCCGCTTTCTTAGG | |
18s rRNA-Fwd | CGTTGATTAAGTCCCTGCCCTT | 18s rRNA |
18s rRNA-Rev | TCAAGTTCGACCGTCTTCTCAG | |
U2snRNA-Fwd | CATCGCTTCTCGGCCTTTTG | U2snRNA |
U2snRNA-Rev | TGGAGGTACTGCAATACCAGG | |
gB-Fwd | GTCCGTGAAGCGGTTCGTGAT | PRV-gB |
gB-Rev | ACAAGTTCAAGGCCCACATCTAC | |
β-actin-Fwd | GTGATCTCCTTCTGCATCCTGTC | β-actin |
β-actin-Rev | CTCCATCATGAAGTGCGACGT | |
lnc641-Fwd | CAGGCATAGAGGGTTAAGGAC | lnc641 |
lnc641-Rev | ACGCTTTGCATGTGGAATTC | |
ALD1114-Fwd | GGTGGGCAAAAGAACTTAGTG | ALD1114 |
ALD1114-Rev | GATAAGAACACGGCTCCCTG | |
lnc1007-Fwd | CTCAGTGGGTTAATGATCCGG | lnc1007 |
lnc1007-Rev | CATATGGAGGTTCCCAGGTTAG | |
ALD8954-Fwd | AAGTGGTACAAGACAGTGTGG | ALD8954 |
ALD8954-Rev | GGAGGTTGGAGGTAAAAGGAC | |
lnc1059-Fwd | TCTTGGGCTCTGCAAATGAG | lnc1059 |
lnc1059-Rev | AAGGCTCCTTCTGTCTTGTTC |