Figure 7

Lnc641 regulates PRV replication by inhibiting type I interferon. A The levels of lnc641, β-actin mRNAs (cytoplasmic RNA positive controls), and U2snRNA (nuclear RNA positive control) in cytoplasmic and nuclear fractions of 3D4/21 cells were determined by qRT-PCR. Results are represented for each gene as means ± SEM from three independent experiments. B, C 3D4/21 cells transfected with si641 and siNC for 36 h, then cells were infected with ZJ01 (0.01 MOI). After 24 h, IFN mRNA levels were detected by qRT-PCR. Meanwhile, the levels of JAK and STAT1 protein were detected by Western blot. D Use of Image Quant software to quantify the protein bands in Fig. 7C Western blot, as normalized to GAPDH. E 3D4/21 cells were transfected with 1 μg pcDNA3.1(+)-641 or pcDNA3.1(+) for 24 h, followed by infection with ZJ01 (0.01 MOI) for 24 h. Then IFN mRNA levels were detected by qRT-PCR. F 3D4/21 cells were transfected with the indicated doses of pcDNA3.1–641 or pcDNA3.1(+) for 24 h, followed by infection with ZJ01 (0.01 MOI) for 24 h, the levels of JAK and STAT1 protein were detected by Western blot. G Use of Image Quant software to quantify the protein bands in F Western blot, as normalized to GAPDH.