Figure 5

Knockdown of lnc641 by siRNAs inhibits PRV replication. A 3D4/21 cells were transfected with two siRNAs (si641-1, si641-2) or negative control (siNC). At 36 h post-transfection, the knockdown efficiency of lnc641 was determined by qRT-PCR. B–E 3D/21 cells placed in 24-well plates were transfected with the two siRNAs for 36 h and then infected with ZJ01 (0.01 MOI). After 24 h, the cell samples were collected to measure the replication of PRV by qRT-PCR (B) and Western blot assays (C). The supernatant was used to measure the viral titers by TCID₅₀ analysis (D). The cells were treated as described previously, and IFA was performed with a primary anti-gB protein monoclonal antibody to analyze the antiviral effect of PRV. Viral gB-protein is green, and nuclei are blue (E). Results are presented as means ± SEM of data from three independent experiments. * P value < 0.05, ** P < 0.01, *** P < 0.0001.