Figure 6
From: Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis
Observation of NETs formation. Bovine neutrophils were evaluated at 30 min after stimulation with live or heat-killed M. bovis (MOI of 100)/PMA in three cows (unstimulated controls, PMA-stimulated, live M. bovis-stimulated, and heat-killed M. bovis-stimulated). DNA was stained using DAPI, and M. bovis was labeled with rhodamine. The white arrow indicates NETs formation. The white bar indicates 16 μm, and representative micrographs are shown.