Figure 6

Electrophoretic mobility shift assay (EMSA) for recombinant Fur_6His binding with the promoter of B739_0173. The DNA of the B739_0173 promoter region and B739_0173-coding region were amplified by PCR, and 50–250 ng of B739_0173 promoter DNA or 250 ng of B739_0173-coding region DNA was mixed with 4 µg of Fur_6His protein in binding buffer at 37 °C for 30 min. The samples were electrophoresed on a gel as described in the “Materials and methods”. (A) The gel was stained with Goldview. (B) The gel was stained with Coomassie Brilliant Blue. The experiment was repeated three times, the B739_0103 promoter region with Fur_6His showed similar results, and a representative image is shown.