Figure 3
The sensitivity of R. anatipestifer CH-1ΔrecA and its derived strains to H2O2-induced oxidative stress. R. anatipestifer CH-1ΔrecApLMF03, R. anatipestifer CH-1ΔrecAΔfurpLMF03 and R. anatipestifer CH-1ΔrecAΔfurpLMF03::fur cells were cultured in GCB medium with no addition (A), with 25 μM EDDHA (B) or with 50 μM EDDHA (C) at 37 °C with shaking cultivation (180 rpm) to the exponential growth phase (OD600 = 1.0–1.5), and the cells were collected and diluted in PBS to 0.5 OD/mL. Then, the cell suspensions were incubated with 0 mM, 5 mM or 10 mM H2O2 at 37 °C for 30 min. The survival rate of each culture was determined as described in the “Materials and methods”. The error bars represent the standard deviations of three independent experiments and three replicate samples for each experiment. Statistical significance was determined using two-way ANOVA (****P < 0.0001, *** P < 0.001, ** P < 0.01).